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Separation of oligosaccharides
Summary
Type:
HPLC
Application #:
102550
Catalog#:
720905.40 - MN HPLC Column, Nucleosil Carbohydrate, 4x250mm
Detection:
fluorescence detection, lex 310 nm, lem 380 nm
Conditions:
eluent A: 200 mM acetic acid (with triethylamine to pH 7.3) - acetonitrile 25:75 (v/v)
eluent B: 200 mM acetic acid (with triethylamine to pH 7.3) - acetonitrile 50:50 (v/v)
gradient 0 - 100% B in 60 min
flow rate 1 ml/min
temperature 28°C
Authors:
Lochnit G
Source:
Biochemical Institute Justus-von-Liebig University
Details
Legend: Chromatogram of a pyridylaminated dextran hydrolyzate, injection volume 100 µl. also see Eur. J. Biochem. 228 (1995) 805 - 816
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